SEPARATION OF ADRENALINE, NORADRENALINE, AND HYDROXYTYRAMINE BY ION EXCHANGE CHROMATOGRAPHY
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Separation of adrenaline, noradrenaline, and hydroxytyramine by ion exchange chromatography.
Methods for the separation of adrenaline, noradrenaline, hydroxytyramine, and dihydroxyphenylalanine (dopa) by employing starch columns (2, 3), paper chromatography (4-7), or countercurrent extraction (8) have limited usage either because of the length of time required for the fractionation or because of the small amounts of material that can be separated. A procedure is described below in whic...
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Enriched stable isotopes of many elements have been widely used in many aspects and such isotopes have been primarily used as the tracer for agricultural and biochemical studies and the availability of the isotopically labeled compounds have been now extensively increasing. Chemical exchange separation of isotopes is based on the equilibrium fractionation of isotopes between two phases, ion exc...
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Shirai, Y. & Ohkubo, T. (1954a). J. Biochem., Tokyo, 41, 337. Shirai, Y. & Ohkubo, T. (1954b). J. Biochem., Tokyo, 41, 341. Storey, I. D. E. (1950). Biochem. J. 47, 212. Storey, I. D. E. & Dutton, G. J. (1955). Biochem. J. 59,279. Storey, I. D. E. & Dutton, G. J. (1956). Proc. 3rd Int. Congr. Biochem., Brussel8, 1955, p. 162. Strominger, J. L., Kalckar, H. M., Axelrod, J. & Maxwell, E. S. (1954...
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Lactate dehydrogenase isoenzymes were partially separated by use of a previously described column technique for creatine kinase [Clin. Chem. 20, 36 (1974)]. Extracts of lactate dehydrogenase-rich tissues were used to evaluate column resolution. Samples layered on mini-columns containing DEAE-Sephadex were eluted with Tris-buffered sodium chloride (100 and 200 mmol/liter). Lactate dehydrogenase ...
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As initial steps in studying the cell-free biosynthesis of guinea pig pancreas ribonuclease, we have developed an ion exchange procedure for its isolation and have analyzed the various subcellular fractions with this technique. This cytological approach was independently applied in studies on the ribonuclease of mouse pancreas (1). Earlier studies on the pancreas of several species have indicat...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64821-5